Western Blot analysis of Ub-K6 in various cell lines using a Ub-K6 Polyclonal antibody (Product # PA5-120610) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.